M.V.Lomonosov Moscow State University
Lomonosovsky av., 31-5
119192 Moscow

Russian Federation

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Project Leader

Prof. Vsevolod Tkachuk Project Leader  Phone: +7-(0)495 9328814  Fax: +7-(0)495 9328814  Contact

Project Staff

Dr. Natalya Kalinina Assoc. Prof./theoretical coordination of the project  Phone: +7-(0)495 9329904  Fax: +7-(0)495 9329904

Dr. Tatiana Kochegura Doctor degree candidate/ practical coordination of the project  Phone: +7-(0)495 9329904  Fax: +7-(0)495 9329904

Dr. Veronika Sysoeva Assoc. Prof./theoretical coordination of the project  Phone: +7-(0)495 9329904  Fax: +7-(0)495 9329904

Dr. Georgiy Sharonov Engineer/coordination of the flow cytometry sorter aspect of work  Phone: +7-(0)495 9329904  Fax: +7-(0)495 9329904

Natalya Iliyashenko Laboratory assistant/ practical coordination of the project  Phone: +7-(0)495 9329904  Fax: +7-(0)495 9329904

Institute Presentation

Scientific staff of the Faculty of Basic Medicine, Lomonosov Moscow State University consists of a highly-competitive, competent specialists, Ph.D. and post-doctoral students in the fields of medicine, cell biology, biochemistry and technical analytical methods. The team possesses a vast knowledge and experience in working with the human adipose tissue derived stem cells (hASC). Our laboratory was the first to provide theoretical and practical exploitation of protocols for extraction of pericyte subpopulations, mesenchymal and endothelial precursors from hASCs and their further differentiation towards osteogenic, adipogenous and chondrogenic lineages. We favor the use of flow cytometry, fluorescence and confocal microscopy methods for the phenotype analysis and subpopulation extraction. We have advanced numerous methods that allow for such functional condition analysis of cells as apoptosis/proliferation, migration rate, secretion, and proangiogenic potential. Our specialty is located within the area of cardiovascular biology. Our routine practice includes such methods as tube assay (capillary-like structure formation) with endothelial cells, cell migration and transmigration assays, as well as hypoxia-induced angiogenesis. According to the demands of the modern medical and biological sciences all of our research is accompanied by the biochemical and molecular-biological analysis of cellular lysates. Apart from the wide spectrum of standard analytical methods, we actively use such techniques as real-time quantitative PCR and PCR array technology, multiplex protein expression analysis. We also possess in-vivo mice models, which are successfully used for research in homing, migration, invasion, angiogenesis stimulation and endocrine function of hASC in vivo.